Profile

• Application of proteome analysis for characterisation of protein composition of biological samples (milk, cell extracts etc.)
• Influence of protein content and composition of milk on milk quality and technological properties
• Proteolytic enzymes in milk – effect on milk protein stability and degradation in relation to functional and organoleptical properties
• Effects of somatic cells on milk quality and stability
• Interactions between proteolysis, oxidation and lipolysis in milk
• Supervision of masters and ph.d. students
• External examiner

The used techniques include among others one- and two-dimensional gel electrophoresis, image analysis, multivariate statistics (Unscrambler), mass spectrometry (MALDI-TOF-TOF), enzymatic assays, use of fluorescent probes for determination of index of proteolysis, electroblotting, immunological methods, zymography, LC Maldi spotting MS/MS, LC-MS.

Actual projects

• Protein composition of raw milk – Influence on the technological properties of milk
• Wound healing effects of bioactive components from milk
• Proteolysis by indigenous milk proteases after modification of substrate protein